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Real-time PCR reagents intended for the qualitative detection of nucleic acid from the common urinary tract infection pathogens in human urine specimens. The assays were established by the full automation method using LabTurbo AIO SP-qPCR system (LabTurbo AIO), LabTurbo stool DNA Mini kit (LSD-480-400) for nucleic acid extraction and LabTurbo AIO Urinary Panel PCR Detection Reagents for qPCR assay.
Features
Improved Detection Sensitvity
Detect nonviable organisms that did not survive transport, organisms difficult to cultivate, and organisms present low numbers.
Reduced Turnaround Time
Finish 16 urinary pathogen detection in 3 hours, then every 16 detections in 1.5 hours. Avoid the 24- 48 hour turn around time by culture method.
Sample-to-result Procedure
Fully integrated for urinary pathogen DNA extraction, PCR setup liquid-handling and real-time PCR pathogen detection with LabTurbo AIO system. No manual steps needed in between.
Great reagent stability and user-friendliness
Store at -20 °C for up to one year. Ship at 4-8 °C. Store at 4-8 °C for up to 3 days after mixing (in-use) for AUT11309611. Avoid more than 5 freeze-thaw cycles. Additionally, we offer pre-aliquoted components for customer convenience in configuration and usage.
Detection Targets
| Gram Positive Bacteria | GramNegative Bacteria | Fungus | |
|---|---|---|---|
| Enterococcus faeclum | Acinetobacter baumannil | Mycoplasma hominis | Candida albicans |
| Staphylococcus aureus | Citrobacter freundii | Proteus mirabilis | Candidaglabrata |
| Staphylococcus saprophyticus | Enterobacter cloacae | Providencia stuartil | Candida tropicalis |
| Streptococcus agalactiae | Escherichia coli | Pseudomonas aeruginosa | |
| Aerococcus urinae | Klebsiella oxytoca | Serratia marcescens | |
| Klebsiella pneumonia | Ureaplasma urealyticum | ||
| Morganella morganlI | Klebslellaaerogenes | ||
Product Description
| Component | Description | Quantity |
|---|---|---|
| Primer/Probe mixture UTI 1, 2, 3, 4, 5, 6, 7, and 8 | For recognization of the specific bacterialnuclei acid targets. | 1 X 240 ul (20 X)for each kind of multiplex master mix. 8 kinds of multiplex master mixes are included. |
| PCR Master Mix (MM) | For polymerase chain reaction | 8 X 1200 ul |
| CFR Positive Control (PC) | DNAmixture ofall thetargets | 1 x 12 ul (50 X) |
| RNase-free water | ForMasterMix preparationa n d negativecontrol | 4 x 1.5 ml |
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