LabTurbo™ AIO Urinary Panel PCR Detection Reagents
LabTurbo™ AIO Urinary Panel PCR Detection Reagents
LabTurbo AIO Urinary Panel PCR Detection Reagents are real-time PCR reagents intended for the qualitative detection of nucleic acid from the common urinary tract infection pathogens in human urine specimens.
The assays were established by the full automation method using LabTurbo AIO SP-qPCR System (LabTurbo AIO), LabTurbo stool DNA Mini kit (LSD480-400) for nucleic acid extraction and LabTurbo AIO Urinary Panel PCR Detection Reagents for qPCR assay.
Advantages and Features
The Optimal qPCR Kit for Urinary Panel PCR Detection
Reduced Turnaround Time
Finish 16 urinary pathogen detection in 3 hours, then every 16 detections in 1.5 hours. Avoid the 24 to 48 hour turnaround time by culture method.
Sample-to-result Procedure
Fully integrated for urinary pathogen DNA extraction, PCR setup liquid-handling and real-time PCR pathogen detection. No manual steps needed in between.
Improved Detection Sensitivity
Detect nonviable organisms that did not survive transport, organisms difficult to cultivate, and organisms present in low numbers.
Target Pathogen
| Gram Positive Bacteria | Gram Negative Bacteria | Gram Negative Bacteria | Fungus |
|---|---|---|---|
| Enterococcus faecalis | Acinetobacter baumannii | Mycoplasma hominis | Candida albicans |
| Enterococcus faecium | Citrobacter freundii | Proteus mirabilis | Candida glabrata |
| Staphylococcus aureus | Enterobacter aerogenes | Proteus vulgaris | Candida tropicalis |
| Staphylococcus saprophyticus | Enterobacter cloacae | Providencia stuartii | |
| Streptococcus agalactiae | Escherichia coli | Pseudomonas aeruginosa | |
| Klebsiella oxytoca | Serratia marcescens | ||
| Klebsiella pneumoniae | Ureaplasma urealyticum | ||
| Morganella morganii |
Kit Content
| Component | Description | Quantity |
|---|---|---|
| Primer/Probe mixture UTI 1, 2, 3, 4, 5, 6, 7, and 8 | For recognization of the specific bacterial nucleic acid targets. | 1 X 120 µl (20 X) for each kind of multiplex master mix. 8 kinds of multiplex master mixes are included. |
| PCR Master Mix (MM) | For polymerase chain reaction | 6 X 1.6 ml (2 X) |
| Dilution buffer (DB) | For diluting the master mix components and master mix preparation. Including the primer/probe mixture for internal control RNaseP gene. (1.85 uM RNaseP forward primer, 1.85 uM RNaseP reverse primer, 0.925 uM Cy5.5 probe) | 6 x 640 ul (1X) |
| Positive Control (PC) | DNA mixture of all the targets | 1 x 12 µl (50 X) |
| RNase-free water | For PCR negative control | 1 x 1.6 ml |